Comparison of different methods for isolating total RNA from bulblet of Fritillaria anhuiensis.
- Author:
Hedong LU
1
;
Tao XUE
;
Yuezhong LI
;
Jianping XUE
;
Wei SHENG
;
Yanfang ZHU
;
Aimin ZHANG
Author Information
- Publication Type:Journal Article
- MeSH: Bentonite; chemistry; DNA, Complementary; analysis; Electrophoresis; Fritillaria; genetics; Guanidines; chemistry; Isothiocyanates; chemistry; Phenol; chemistry; Plant Roots; genetics; Plants, Medicinal; genetics; RNA, Plant; analysis; isolation & purification; Reverse Transcriptase Polymerase Chain Reaction; Sodium Dodecyl Sulfate; chemistry; Time Factors
- From: China Journal of Chinese Materia Medica 2012;37(6):781-784
- CountryChina
- Language:Chinese
-
Abstract:
OBJECTIVETo optimize a simple and effective method for total RNA extraction from bulblet of Fritillaria anhuiensis.
METHODFour methods, i. e. guanidine isothiocyanate, bentonite, modified SDS/phenol and the RNAiso plus, were used to extract total RNA from bulblet of F. anhuiensis. Then the results of the extraction were compared and analyzed by electrophoresis detection and RT-PCR verification.
RESULTThe total RNA extracted by bentonite method were clear and no dispersion, the integrity of the RNA was well, and there was no obvious contamination with DNA and other impurities, was suitable for RT-PCR test.
CONCLUSIONThe bentonite method is quick, economic, and efficient for total RNA extraction from bulblet of F. anhuiensis.
