Simultaneous determination of four components in sini tang by HPLC.
- Author:
Xiao LIU
1
;
Linqian FAN
;
Hao CAI
;
Baochang CAI
Author Information
- Publication Type:Journal Article
- MeSH: Aconitine; analogs & derivatives; analysis; isolation & purification; Anti-Inflammatory Agents; analysis; isolation & purification; Catechols; analysis; isolation & purification; Chromatography, High Pressure Liquid; methods; Drugs, Chinese Herbal; chemistry; Fatty Alcohols; analysis; isolation & purification; Flavanones; analysis; isolation & purification; Glucosides; analysis; isolation & purification; Glycyrrhizic Acid; analysis; isolation & purification; Medicine, Chinese Traditional; Reproducibility of Results
- From: China Journal of Chinese Materia Medica 2012;37(6):803-805
- CountryChina
- Language:Chinese
-
Abstract:
OBJECTIVETo develop an HPLC method for determining four components in Sini Tan, benzoylmesaconine, liquiritin, glycyrrhizic acid and 6-gingerol.
METHODThe Hypersil BDS column was adopted with gradient elution program at a flow rate of 1.0 mL x min(-1) and the detection wavelength of 235 nm.
RESULTBenzoylmesaconine, liquiritin, glycyrrhizic acid and 6-gingerol showed good separation, with the linear range of 0.006-0.12, 0.021-0.42, 0.012-0.24 and 0.018-0.36 g x L(-1), respectively. Their average recoveries were 99.3%, 96.9%, 100% and 100%, respectively; and RSD of the above four components were 1.5%, 0.6%, 1.3% and 2.1%, respectively.
CONCLUSIONThe method is proved to be so easy and accurate and practical that it can be used to determine the four components in Sini Tang.
