Simultaneous determination of chlorogenic acid and vitexin 2"-rhamnoside in Crataegi Fructus extracts by HPLC.
- Author:
Yajun HE
1
;
Juan SU
;
Qian YANG
;
Hui LIU
;
Weidong ZHANG
Author Information
- Publication Type:Journal Article
- MeSH: Apigenin; analysis; isolation & purification; Chlorogenic Acid; analysis; isolation & purification; Chromatography, High Pressure Liquid; Crataegus; chemistry; Drugs, Chinese Herbal; chemistry; Fruit; chemistry; Medicine, Chinese Traditional; Plant Extracts; chemistry; Plants, Medicinal; chemistry; Reproducibility of Results
- From: China Journal of Chinese Materia Medica 2012;37(6):829-831
- CountryChina
- Language:Chinese
-
Abstract:
OBJECTIVETo establish a determination method for contents of chlorogenic acid and vitexin 2"-rhamnoside in Crataegi Fructus extracts by HPLC.
METHODThe chromatographic column was SHISEIDO CAPCELL PAK C18 (4.6 mm x 250 mm, 5 microm); mobile phase was methanol(A) and THF-acetic acid-water (10:2:76, B), with gradient elution, flow speed was 1.0 mL x min(-1); detection wavelength was 330 nm; temperature of column was 25 degrees C.
RESULTChlorogenic acid and vitexin 2"-rhamnoside showed good linear relationships within the ranges of 1.34-164.8 and 1.18-148.7 mg x L(-1), with the recovery rates being 100.4% and 98.8%, and RSD being 1.5% and 1.3% respectively.
CONCLUSIONThe present method is so simply, accurate and highly repeatable that it can be used to effectively determine the contents of chlorogenic acid and vitexin 2"-rhamnoside in Crataegi Fructus extracts.
