Fingerprint of Angelica polymorpha by HPLC.
- Author:
Jinqing LU
1
;
Qin WANG
;
Jiaqi XU
;
Dongli HE
;
Xiaoyun RUAN
;
Xiaoyan HU
Author Information
- Publication Type:Journal Article
- MeSH: Angelica; chemistry; China; Chromatography, High Pressure Liquid; methods; Drugs, Chinese Herbal; chemistry; isolation & purification; Furocoumarins; chemistry; Plant Roots; chemistry; Plants, Medicinal; chemistry; Quality Control; Reference Standards; Reproducibility of Results
- From: China Journal of Chinese Materia Medica 2012;37(6):832-835
- CountryChina
- Language:Chinese
-
Abstract:
OBJECTIVETo establish the HPLC fingerprint of Angelica polymorpha.
METHODThe 10 batches of A. polymorpha were measured by HPLC with isoimperatorin as a reference substance and the chromatographic experients were performed on Kromasil 100A C18 column (4.6 mm x 250 mm, 5 microm), eluted with acetonitrile and water as mobile phase in gradient mode. The flow rate was 1.0 m x min(-1) and the detection wavelength was 254 nm.
RESULTThe common mode of the HPLC fingerprints were set up. There were 8 common peaks in the fingerprint of 10 samples, and the similarity of the 10 samples was more than 0.9.
CONCLUSIONThe method is simple, accurate and have a good reaptability. The quality of A. polymorpha can be controlled effectively by the HPLC fingerprint.
