Effect of zedoary oil for cat D and cat K expression in A549 cell line.
- Author:
Changfu YANG
1
;
Chunfang HUANG
;
Xiaofang SUN
;
Jianzhao NIU
;
Jifeng WANG
Author Information
- Publication Type:Journal Article
- MeSH: Animals; Blotting, Western; Cathepsin D; metabolism; Cathepsin K; metabolism; Cell Line, Tumor; Collagen; metabolism; Curcuma; chemistry; Gene Expression Regulation, Neoplastic; drug effects; Plant Oils; isolation & purification; pharmacology; Up-Regulation
- From: China Journal of Chinese Materia Medica 2012;37(6):842-846
- CountryChina
- Language:Chinese
-
Abstract:
OBJECTIVETo explore the Zedoary oil on A549 cell line of collagen deposition cat D and cat K expression.
METHODThe A549 cell line were treat by Zedoary oil on four different concentrations (0, 40, 80, 120 mg x L(-1)) in different time. Dynamic changes of collagen in A549 cell using Picric-sirius red method. Cat D and Cat K expression of level were detected by using western blot.
RESULTThe collagen content showed that Zedoary oil had an inhibitory effect on the deposition of A549 cells. The results of western blot showed that the expression of cat D and cat K were up-regulated significangly in A549 cells of Zedoary oil groups compared with that in controls.
CONCLUSIONA549 cell of collagen deposition were reduced by Zedoary oil. The effects may due to the up-regulation of cat D and cat K.
