Determination of brucine and strychnine in rat after cutaneous administration of semen strychni niosome gel by LC-MS/MS.
- Author:
Jingya LI
1
;
Fengru MIAO
;
Li LIN
;
Dan ZHU
;
Chengren LIN
;
Jianxun LIU
Author Information
- Publication Type:Journal Article
- MeSH: Administration, Cutaneous; Analgesics; analysis; pharmacokinetics; Animals; Chromatography, Liquid; Convulsants; analysis; pharmacokinetics; Female; Gels; chemistry; Liposomes; chemistry; Male; Plants, Medicinal; chemistry; Rats; Rats, Sprague-Dawley; Seeds; chemistry; Semen; chemistry; Specific Pathogen-Free Organisms; Strychnine; analogs & derivatives; analysis; pharmacokinetics; Strychnos nux-vomica; chemistry; Tandem Mass Spectrometry
- From: China Journal of Chinese Materia Medica 2012;37(6):853-857
- CountryChina
- Language:Chinese
-
Abstract:
OBJECTIVEA sensitive liquid chromatography-tandem mass spectrometric (LC-MS/MS) method has been developed and validated for the determination of brucine and strychnine in rat plasma.
METHODSamples were extracted by ethyl acetate-n-butanol (7: 3). Chromatographic separation was operated on ZORBAX XDB-C18 column with gradient elution of acetonitrile-methanol-water (0.05% acetic acid and 10 nmol x L(-1) ammonium formate contained), followed by LC-MS/MS in positive electrospray ionization. Quantification was carried out on multiple reaction monitoring (MRM) of the transition m/z 395.2/324.2, m/z 335.2/184.2 and m/z 199.1/171.1 for brucine, strychnine and tacrine (internal standard), respectively.
RESULTThe method was linear in the range of 0.195-100 and 0.07840 microg x L(-1) for brucine and strychnine, with coefficient correlation 0.994 and 0.996 respectively. The recoveries of extraction were 78.9% - 102.4% for brucine and 95.2% - 106.1% for strychnine. Precision, accuracy, stability and matrix effect of the analytes met the requirement. The method was applied to a pharmacokinetic study of brucine and strychnine after cutaneous administration of Semen Strychni niosome gel. The C(max) were (26.20 +/- 5.81) and (12.50 +/- 3.00) microg x L(-1) while the AUC(0-infinity), were (193.75 +/- 39.43) and (98.25 +/- 28.54) microg x h x L(-1) of the two components.
CONCLUSIONWe conclude that the niosomes may reduce the systemic exposures and prolong the local release of brucine and strychnine.
