Determination of trolline in flowers of Trollius chinensis by HPLC.
- Author:
Rufeng WANG
1
;
Qun MA
;
Jifeng YANG
Author Information
1. School of Chinese Medicine, Beijing University of Chinese Medicine, Beijing 100102, China. waggrufemg@tsimghua.org.cn
- Publication Type:Journal Article
- MeSH:
Alkaloids;
analysis;
Chromatography, High Pressure Liquid;
methods;
Flowers;
chemistry;
Ranunculaceae;
chemistry;
Reproducibility of Results
- From:
China Journal of Chinese Materia Medica
2012;37(2):247-249
- CountryChina
- Language:Chinese
-
Abstract:
An HPLC method was developed for determinantion of trolline in the flowers of Trollius chinensis commercially available in China. The HPLC analysis was conducted on an Agilent C18 ODS column (4.6 mm x 250 mm, 5 microm) with acetonitrile-0.5% acetic acid solution (gradient: 0-30 min, 2:98-100:0) as mobile phase at a flow rate of 1.0 mL x min(-1). The detecting wavelength was at 258 nm. Linearity of trolline was good in the range of 0.1380-1.2410 microg and the content of trolline in nine batches of the flowers of T. chinensis commercially available in China was ranged from 0.05% to 0.11%. This method is good in terms of precision, accuracy and repeatability and can be used for the quantitative determination of trolline.
