Simultaneous determination of three homoisoflavonoids in Ophiopogon japonicus by HPLC.
- Author:
Pintao ZENG
1
;
Hui ZHOU
;
Yimin ZHENG
;
Xiuying XU
;
Shanquan FU
Author Information
1. School of Pharmacy and Bioengineering, Chongqing University of Technology, Chongqing 400050, China.
- Publication Type:Journal Article
- MeSH:
Chromatography, High Pressure Liquid;
methods;
Drugs, Chinese Herbal;
analysis;
Isoflavones;
analysis;
Ophiopogon;
chemistry
- From:
China Journal of Chinese Materia Medica
2012;37(1):71-74
- CountryChina
- Language:Chinese
-
Abstract:
Three homoisoflavonoids, 6-aldehydo-3-ophiopogonanone A, methyl ophiopogonanone A and ophiopogonanone A from Ophiopogon japonicus were analyzed simultaneously by HPLC with acetonitrile-water containing 0.5% H3 PO4 (58:42) as the mobile phase, and the detection wavelength was set at 296 nm (0-14 min) and 275 nm (14-22 min). The mean recoveries of three homoisoflavonoids were 99.41%-99.86% (RSD 0.82%-1.05%). The linear response ranges of. 6-aldehydo-3-ophiopogonanone A, methyl ophiopogonanone A and ophiopogonanone A were 0.165-0.990 microg (r = 0.999 9), 0.153-0.918 microg (r = 0.999 9), and 0.270-1.620 microg (r = 0.999 9), respectively. This method was certified to be accurate and reliable and can be used for quality control of O. japonicus.
