Developing a third-generation anti-HCV enzyme immunoassay using recombinant proteins expressed in Escherichia coli.
- Author:
Dat Tuan Do
- Publication Type:Journal Article
- Keywords:
Hepatitis C
- MeSH:
Hepatitis C;
Escherichia coli
- From:Journal of Preventive Medicine
2000;10(4):11-18
- CountryViet Nam
- Language:Vietnamese
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Abstract:
4 hepatitis C virus (HCV) recombinant proteins with GST (glutathione S-transferase) structure expressed in Escherichia coli was purified for developing a third-generation anti-HCV Enzyme Immunoassay - GST anti-HCV EIA-3. This diagnostic kit was able to detect all HCV RIBA-3 positive sera or HCV RIBA-3 indeterminate (only one reactive band) and PCR positive sera in HCV panels from Russian and Indonesian blood donors, US HCV genotype panel. With HCV RIBA-3 indeterminate specimens, this test is strongly reactive (at least 2 antigens) while core or NS3 band are positive and non-reactive while NS4 or NS5 band are positive.
