Expression and significance of GCS gene in human pancreatic cancer cell SW1990 and its drug-resistant sublines.
- Author:
Guang-bo BU
1
;
Yu-pei ZHAO
;
Ge CHEN
;
Tai-ping ZHANG
;
Li-jun LI
;
Yuan-de WU
Author Information
- Publication Type:Journal Article
- MeSH: Cell Line, Tumor; Drug Resistance, Neoplasm; genetics; Gene Expression; Glucosyltransferases; biosynthesis; genetics; Humans; Immunoblotting; Pancreatic Neoplasms; enzymology; genetics; pathology; RNA, Messenger; genetics; Reverse Transcriptase Polymerase Chain Reaction
- From: Chinese Journal of Surgery 2006;44(19):1342-1344
- CountryChina
- Language:Chinese
-
Abstract:
OBJECTIVETo study the expression and significance of GCS gene in human pancreatic cancer cell line SW1990 and its drug-resistant sublines.
METHODSSW1990 and its drug-resistant sublines, SW1990/FU, SW1990/ADM and SW1990/GEM were cultured in vitro. CCK-8 (Cell Counting kit-8) was used to detect the drug resistance of the sublines. Relative quantitation of GCS mRNA expression was evaluated by real-time PCR and Western blot was adopted to evaluate the expression of GCS protein.
RESULTSThe drug resistance indexes of SW1990/FU, SW1990/ADM and SW1990/GEM were 339.7, 11.9 and 56.6, respectively. The GCS mRNA and protein were expressed in all SW1990 and its drug-resistant sublines. There was a higher expression of GCS mRNA in all the sublines and a significant difference of GCS protein expression was detected in SW1990/ADM and SW1990/GEM compared with SW1990.
CONCLUSIONSGCS gene is expressed in SW1990 and its drug-resistance sublines. The high expression of GCS protein in SW1990/ADM and SW1990/GEM might be one reason of resistance to ADM and GEM in the sublines.
