Nuclear factor-kappaB expression in peripheral blood polymorphonuclear leukocytes of patients with acute pancreatitis and the preventive effectiveness of pyrrolidine dithiocarbamate on NF-kappaB in vitro.
- Author:
Jia-hua ZHOU
1
;
Huan-song LI
;
Hai-tao ZHU
;
Feng-Chen WANG
;
De-tong YANG
Author Information
- Publication Type:Journal Article
- MeSH: Acute Disease; Adult; Aged; Aged, 80 and over; Female; Humans; Male; Middle Aged; NF-kappa B; biosynthesis; blood; Neutrophils; drug effects; metabolism; Pancreatitis; metabolism; Pyrrolidines; pharmacology; Thiocarbamates; pharmacology
- From: Chinese Journal of Surgery 2006;44(19):1345-1348
- CountryChina
- Language:Chinese
-
Abstract:
OBJECTIVETo investigate the expression of NF-kappaB in peripheral blood polymorphonuclear leukocyte (PMN) of acute pancreatitis (AP) and to assess the preventive effectiveness of pyrrolidine dithiocarbamate (PDTC) on NF-kappaB in vitro.
METHODSNineteen patients and 16 healthy individuals as control were enrolled in this study. The expression of NF-kappaB in PMNs was determined by gel electrophoretic mobility shift assay (EMSA). Routine clinical examination results and computed tomography findings of AP were recorded in all patients.
RESULTSThe PMNs from the patients with AP showed higher levels of NF-kappaB activities than those from control subjects (P < 0.01), severe acute pancreatitis (SAP) group showed much higher than mild acute pancreatitis (MAP) group (P < 0.05). In vitro, PDTC could reduce the NF-kappaB activity in PMNs of patients with AP, and its effectiveness at 2 mmol/L was stronger than at 1 mmol/L (P < 0.05). The PMNs from control subjects pretreated with 2 mmol/L PDTC before stimulation with the plasma from patients with SAP showed lower levels of NF-kappaB activities than did those untreated (P < 0.05).
CONCLUSIONThe NF-kappaB activation in peripheral blood PMNs participate in the course of acute pancreatitis and can be inhibited by PDTC in vitro.
