Effects of Bushen Zhuangjin Decoction containing serum on the apoptosis of chondrocytes induced by mechanics stimulus.
- Author:
Guo-Shun ZHOU
1
;
Xiong-Feng LI
;
Guo-Hua GUAN
Author Information
- Publication Type:Journal Article
- MeSH: Animals; Apoptosis; drug effects; Cartilage, Articular; drug effects; Cells, Cultured; Chondrocytes; cytology; drug effects; Drugs, Chinese Herbal; pharmacology; Flow Cytometry; Interleukin-1beta; analysis; Nitric Oxide; analysis; Rabbits; Serum
- From: Chinese Journal of Integrated Traditional and Western Medicine 2012;32(6):789-792
- CountryChina
- Language:Chinese
-
Abstract:
OBJECTIVETo study the effects of Bushen Zhuangjin Decoction (BZD) containing serum on the apoptosis of chondrocytes induced by mechanics stimulus.
METHODSThe BZD containing serum was extracted. The chondrocyte nutritive media was divided into 3 groups, i.e., the common nutritive medium group, the blank rabbit serum medium group, and the BZD nutritive medium group. The apoptosis of chondrocytes was induced by continuing mechanics stimulus in 24 h. Then the chondrocytes were collected. The apoptosis rate of chondrocytes was determined by flow cytometry. The contents of interleukin 1beta (IL-1beta) and nitric oxide (NO) in the corresponding media were determined.
RESULTSThe apoptosis of chondrocytes in the BZD nutritive medium group (19.55 +/- 7.98)% was lower than that of the common nutritive medium group (39.32 +/- 13.45)% and the blank rabbit serum medium group (37.87 +/- 9.67)%, showing statistical difference (P < 0.05). The contents of IL-1beta and NO were also lower in the BZD nutritive medium group with statistical difference when compared with those of the other two groups (P < 0.05).
CONCLUSIONBZD containing serum could protect mechanics stimulus induced apoptosis of chondrocytes.
