Effects of glycyrrhetinic acid on sodium ion channel currents of rats' ventricular myocardial cells.
- Author:
Ji-Yuan YANG
1
;
Hong-Jin WU
;
De-Lin WU
Author Information
- Publication Type:Journal Article
- MeSH: Animals; Glycyrrhetinic Acid; pharmacology; Heart Ventricles; cytology; metabolism; Male; Myocytes, Cardiac; drug effects; physiology; Patch-Clamp Techniques; Rats; Rats, Sprague-Dawley; Sodium Channels; drug effects; physiology
- From: Chinese Journal of Integrated Traditional and Western Medicine 2012;32(7):944-947
- CountryChina
- Language:Chinese
-
Abstract:
OBJECTIVETo study the effects of glycyrrhetinic acid (GA) on the sodium ion channel currents (I(Na)) of rats' ventricular myocardial cells, and to explore its anti-arrhythmic mechanisms at the ion channel level.
METHODSSingle ventricular myocardial cells was isolated from SD rats. The whole cell patch clamp was used to record the effects of GA on I(Na) of rats' ventricular myocardial cells.
RESULTSGA could inhibit I(Na) of rats' ventricular myocardial cells dose-dependently. GA at 1, 5, and 10 micromol/L decreased I(Na) of rats' ventricular myocardial cells from (-4.26 +/- 0.15) nA to (-3.54 +/- 0.10) nA, (-2.19 +/- 0.09) nA, and (-1.25 +/- 0.08) nA, respectively. GA at 1, 5, and 10 micromol/L inhibited I(Na) by 16.08% +/- 2.3%, 50.82% +/- 3.56%, and 75.98% +/- 5.12%, showing statistical difference when compared with the control group (P < 0.05). GA at 10 micromol/L shifted I(Na) current-voltage curve more positively, but the activation potential and the peak potential were not changed.
CONCLUSIONGA inhibited the I(Na) of rats' ventricular myocardial cells dose-dependently, which was possibly associated with its antiarrhythmia effects.
