Rat kidney mitochondrial damage induced by acute cadmium administration.
- Author:
Xiao-Hai PAN
1
;
Kai SUN
;
Jia-Hu PAN
;
Tai-Yi JIN
Author Information
- Publication Type:Journal Article
- MeSH: Animals; Cadmium; toxicity; Kidney; drug effects; metabolism; Male; Membrane Potential, Mitochondrial; Mitochondria; drug effects; metabolism; pathology; Oxygen; metabolism; Rats; Rats, Sprague-Dawley; Reactive Oxygen Species; metabolism; Toxicity Tests, Acute
- From: Chinese Journal of Industrial Hygiene and Occupational Diseases 2010;28(5):345-349
- CountryChina
- Language:Chinese
-
Abstract:
OBJECTIVETo evaluate effect of acute cadmium administration on mitochondria from rat kidney.
METHODS24 male Sprague-Dawley (SD) rats were randomly divided into four groups. Four groups of rats were injected with saline, 0.6, 1.2, and 1.8 mg/kg weight subcutaneously, once daily, for 5 days, respectively. Ultrastructural change of rat kidney mitochondria was observed, and respiration function, membrane potential, mitochondria swelling, and superoxide level were determined.
RESULTSUltrastructural changes included matrix vacuolation, swelling and condensation of mitochondria. In group of 1.8 mg/kg body weight, the oxygen consumption rate during state 3 respiration [(6.25 +/- 0.61) nmol/L O2 x min(-1) x mg(-1)] and RCR value (2.45 +/- 0.23) were significantly lower than those of control group [(9.66 +/- 1.16) nmol/L O2 x min(-1) x mg(-1)] (P < 0.05), indicating respiration inhibition. The membrane potential and superoxide level of the same group were 85.89% +/- 3.82% and 116.33% +/- 3.06% of control values (P < 0.05), respectively.
CONCLUSIONSAcute cadmium administration can cause rat kidney mitochondrial damage in a dose-effect manner, including inhibition of respiration, dissipation of membrane potential, swelling of mitochondria matrix. Such damage might be related to the increase of mitochondrial free radical.
