A study on evaluation of antigenicity of hepatitis C virus recombinant proteins basing on serum panel from Vietnamese blood donors
- Author:
Dat Tuan Do
- Publication Type:Journal Article
- Keywords:
hepatitis C
- MeSH:
Hepatitis C virus;
Escherichia coli
- From:Journal of Preventive Medicine
2000;10(4):5-13
- CountryViet Nam
- Language:Vietnamese
-
Abstract:
A serum panel from Vietnamese blood donors including 225 positive and 300 negative sera has been used to evaluate antigenicity of 8 hepatitis C virus (HCV) recombinant proteins cloned and expressed in Escherichia coli c22-3, c33/1b, mosaic NS4, NS5a/1b proteins presented identical results while third-generation HCV recombinant immunoblot assay (RIBA-3) sera were positive. All recombinant proteins highly detected HCV RNA positive sera known genotype (80-95%). NS3-16/6a demonstrated greater reactivity than other proteins, indeed a good protein (c33c/1b) in the same region while HCV genotype was 6a (specific HCV genotype in Vietnam).
