Computer construction and analysis of protein models of the mutant gammaD-crystallin gene.
- Author:
Ke YAO
1
;
Zhao-hui SUN
;
Xing-chao SHENTU
;
Kai-jun WANG
;
Jian TAN
Author Information
- Publication Type:Journal Article
- MeSH: Animals; Cattle; Computer Simulation; Hydrogen Bonding; Models, Molecular; Mutation; Protein Structure, Tertiary; gamma-Crystallins; chemistry; genetics; physiology
- From: Chinese Medical Journal 2005;118(9):738-741
- CountryChina
- Language:English
-
Abstract:
BACKGROUNDGammad-crystallin plays an important role in human cataract formation. Being highly stable, gammaD-crystallin proteins are composed of two domains. In this study we constructed and analyzed protein models of the mutant gammaD-crystallin gene, which caused a special fasciculiform congenital cataract affecting a large Chinese family.
METHODSgammaD-crystallin protein structure was predicted by Swiss-Model software using bovine gammaD-crystallin as a template and Prospect software using human betab2-crystallin as a template. The models were observed with a Swiss-Pdb viewer.
RESULTSThe mutant gammaD-crystallin structure predicted by the Swiss-Model software showed that proline23 was an exposed surface residue and P23T change made a decreased hydrogen bond distance between threonine23 and asparagine49. The mutant gammaD-crystallin structure predicted by the Prospect software showed that the P23T change exerted a significant effect on the protein's tertiary structure and yielded hydrogen bonds with aspartic acid21, asparagine24, asparagine49 and serine74.
CONCLUSIONThe mutant gammaD-crystallin gene has a significant effect on the protein's tertiary structure, supporting that alteration of gamma-crystallin plays an important role in human cataract formation.
