ALK fusion gene assessment by fully automatic immunohistochemistry in non-small cell lung cancer.
- Author:
Lei GUO
1
;
Xiuyun LIU
1
;
Tian QIU
1
;
Yun LING
1
;
Ling SHAN
1
;
Yongqiang XIE
2
Author Information
- Publication Type:Journal Article
- MeSH: Adenocarcinoma; genetics; metabolism; Adult; Aged; Aged, 80 and over; Carcinoma, Non-Small-Cell Lung; genetics; metabolism; Female; Gene Rearrangement; Humans; Immunohistochemistry; In Situ Hybridization, Fluorescence; Lung Neoplasms; genetics; metabolism; Male; Middle Aged; Oncogene Proteins, Fusion; genetics; metabolism; Sensitivity and Specificity
- From: Chinese Journal of Pathology 2014;43(2):95-98
- CountryChina
- Language:Chinese
-
Abstract:
OBJECTIVETo evaluate the sensitivity and specificity of fully automated immunohistochemistry (IHC), with comparison to FISH, in the detection of EML4-ALK rearrangement in lung adenocarcinoma (ADC); and the use of IHC as a pre-screening tool.
METHODSA total of 404 paraffin-embedded NSCLC samples from surgical resections were tested by IHC with Ventana anti-ALK rabbit monoclonal antibody (D5F3) and ultrasensitive detection kit. ALK rearrangement was further confirmed by FISH.
RESULTSTwenty-nine of 404 lung ADCs (7.2%) were positive for ALK by IHC. ALK positive tumor cells demonstrated strong and diffused granular cytoplasmic staining. All the ALK IHC-positive cases were confirmed to harbor ALK rearrangement by FISH. None of the ALK IHC-negative cases was FISH-positive.
CONCLUSIONSIHC can effectively detect ALK rearrangement in lung cancer. It may provide a reliable and cost-effective diagnostic approach in routine pathologic laboratories for the identification of suitable candidates for ALK-targeted therapy.