A rapid screening program on the resistance to streptomycin and ethambutol in Mycobacterium tuberculosis isolates by PCR melting curve analysis
10.3760/cma.j.issn.0254-6450.2012.05.018
- VernacularTitle:PCR熔解曲线法筛查结核分枝杆菌链霉素和乙胺丁醇耐药性
- Author:
Feng WANG
1
;
Si-Yu HU
;
Jing GUI
;
Yun-Yong CUI
;
Xiao-Li LIU
;
Qing-Ge LI
Author Information
1. 深圳市慢性病防治中心
- Keywords:
Mycobacterium tuberculosis;
Drug resistance;
Streptomycin;
Ethambutol
- From:Chinese Journal of Epidemiology
2012;33(5):525-528
- CountryChina
- Language:Chinese
-
Abstract:
Objective To evaluate the effects of PCR melting curve analysis assay on a rapid screening program regarding the resistance of Mycobacterium tuberculosis (MTB) clinical isolates to streptomycin and ethambutol.Methods A total of 331 clinical isolates of MTB had been collected since 2007-2009 in Shenzhen.Mutations at codon 306,378-380,406 and 497 of embB gene,codon 43,88 of rpsL gene,and 513-517,905-908 region of rrs gene were detected by PCR melting curve analysis.Results were compared with that of conventional drug susceptibility test.Results Compared to drug susceptibility test,sensitivity,specificity and accuracy for streptomycin resistance were 78.6％,90.1％ and 86.7％,respectively while 83.0％,93.3％ and 91.8％,respectively for ethambutol resistance detected by PCR melting curve analysis.PCR melting curve method was in good agreement with drug susceptibility test.Conclusion PCR melting curve analysis on genetic regions associated with resistance to streptomycin and ethambutol seemed to be a rapid,specific and closed-tube method so it could be used for detection oftreptomycin and ethambutol resistance in MTB.
