Identification and sequencial analysis on rabies virus isolated from a donkey
10.3760/cma.j.issn.0254-6450.2012.06.013
- VernacularTitle:1株驴源狂犬病毒的分离鉴定及N、G基因序列分析
- Author:
Ting-Bo XIE
1
;
Hua YU
;
Jie WU
;
Si-Jia HUANG
;
Ge-Lin XU
;
Jia-Xin YAN
;
Bin YU
;
Dun-Jin ZHOU
Author Information
1. 湖北省武汉市生物制品研究所
- Keywords:
Rabies virus;
Genetic characteristics;
Donkey
- From:
Chinese Journal of Epidemiology
2012;33(6):602-605
- CountryChina
- Language:Chinese
-
Abstract:
Objective To identify and analyze the genetic characteristics of nucleoprotein (N) and glycoprotein (G) genes of rabies virus (RABV) isolated from a donkey in Wuhan.N gene and G gene of the virus were compared with other representative street strains isolated around Hubei areas as well as the vaccine strains used in China and abroad.Methods RABV in brain tissue of a donkey was detected by direct immunofluorescent method and then inoculated in suckling mice to observe the incidence of rabies.Brain samples of the donkey and infected suckling mice were detected by ELISA.The N gene and G gene fragment of the isolated RABV were amplified by RT-PCR and cloned into pMD18-T vector for sequencing and genetic analysis.Results RABVs were detected in both donkey brain and suckling mice brain samples.The N gene and G gene nueleotide homology of RABV isolated from the donkey with other representative street strains found around Hubei areas as well as vaccine strains used in China and abroad were 85.7％-99.1％ and 82.2％-99.7％,and the deduced amino acid identity were 95.6％-99.8％ and 87.8％-99.4％,respectively.Conclusion Novel RABV was successfully identified and isolated from a donkey and showed close relationship to the representative street strains found around Hubei areas as well as vaccine strains used in China through genetic analysis.
