Study on the Fast Testing Strategy for identifying the wild poliovirus Ⅰ
10.3760/cma.j.issn.0254-6450.2012.07.018
- VernacularTitle:Ⅰ型脊髓灰质炎野病毒实时荧光RT-PCR快速检测方法的评价
- Author:
Cheng GONG
1
;
Ming LUO
;
Meng CHEN
;
Tie-Gang ZHANG
;
He-Run ZHANG
;
Yu-Mei WANG
;
Ren-Qing LI
;
Mei DONG
;
Wei-Xin CHEN
;
Li-Juan CHEN
Author Information
1. 北京市疾病预防控制中心
- Keywords:
Poliovirus;
Poliomyelitis,wild poliovirus;
Real time RT-PCR
- From:
Chinese Journal of Epidemiology
2012;33(7):726-729
- CountryChina
- Language:Chinese
-
Abstract:
Objective To explore the Fast Testing Sstrategy (FTS) for wild poliovirus Ⅰ (WP1).Methods Epidemiological investigations were carried out on 671 students from WP1 epidemic areas in China.A set of real time RT-PCR assays,including panenterovirus testings (PE) assay,poliovirus serotypings(PS) assay and the assay distinguishing wild strain from vaccine strain of poliovirus Ⅰ (DWV) were introduced into the screening program for WPV1 to replace the conventional RT-PCR,recommended by the China National Polio Laboratory (GNPL).Additionally,sensitivities of all the assays were assessed by poliovirus type Ⅰ to Ⅲ (Sabin stain) and the isolated WPV I.Results ( 1 ) 33 non-poliovirus enterovirus (NPEV) cases were detected,with 16 polio vaccinerelated cases including 5 polio Ⅰ,1 polio Ⅱ,3 polio Ⅲ,1 polio Ⅰ +Ⅱ,4 polio Ⅰ + Ⅲ and 2 polio Ⅰ + Ⅱ + Ⅲ.Three WPV 1 cases were also detected in this study and confirmed by GNPL.(2) For polio virus vaccine strain,sensitivities of the set of real time RT-PCR assays ranged from 1 to 100 times than that of the in-housc RT-PCR assay.The sensitivities of PE and PS assays for the detection of polio Ⅱ were 100 times than that of the RT-PCR assay and the sensitivity of DWV assay used for the detection of polio Ⅰ were 10 times than that of the RT-PCR assay.For WPV1,the sensitivity of three real time RT-PCR was 10 times higher than that of the RT-PCR assay.Conclusion The novel FTS for WPV I suggested by this study would include PE,PS and DWV.It not only could greatly shorten the testing time but also more sensitive than the RT-PCR and suited for emergency detection for WPV1.
