Association of SPO11 and GST gene polymorphisms with idiopathic male infertility in ethnic Han Chinese.
- Author:
Zhanqi FENG
1
;
Zhian JING
;
Hongyan LIU
;
Shixiu LIAO
;
Liangjie GUO
;
Changqing MAO
;
Yanjun LIU
;
Hui WU
;
Jiangtao GAO
Author Information
- Publication Type:Journal Article
- MeSH: Adult; Alleles; Asian Continental Ancestry Group; genetics; Base Sequence; China; Endodeoxyribonucleases; genetics; Gene Frequency; Genetic Predisposition to Disease; ethnology; genetics; Genotype; Glutathione S-Transferase pi; genetics; Glutathione Transferase; genetics; Humans; Infertility, Male; enzymology; ethnology; genetics; Linkage Disequilibrium; Male; Mutation; Odds Ratio; Polymorphism, Genetic; Sequence Analysis, DNA
- From: Chinese Journal of Medical Genetics 2015;32(6):866-870
- CountryChina
- Language:Chinese
-
Abstract:
OBJECTIVETo explore the possible roles of polymorphisms of SPO11 and glutathionine S-transferase (GST) genes in idiopathic male infertility in a ethnic Han Chinese population from Henan.
METHODSMultiplex PCR and DNA sequencing were performed to determine the SPO11 c.517C>T(rs28368082) and GST genes (GSTM1, GSTT1, GSTP1) polymorphisms in 216 idiopathic male infertility cases and 198 normal samples.
RESULTSThe frequencies of the SPO11 CC and CT genotypes were 87.5% (189/216) and 12.5% (27/216) in the patients, and 97.5% (193/198) and 2.5% (5/198) in the controls, respectively. The frequencies of SPO11 CC and CT genotypes, the A>G transition at nucleotide 313 in the exon 5 of the GSTP1 gene, and the frequencies of combined genotypes GSTM1 (-/-), GSTT1 (+/+), GSTP1 (AA) and SPO11 (CT) were significantly different between the two groups (P<0.05).
CONCLUSIONThe rs28368082 polymorphism of the SPO11 gene, the A>G transition at nucleotide 313 in the exon 5 of the GSTP1 gene, and the combined genotypes of GSTM1 (-/-), GSTT1 (+/+), GSTP1 (AA) and SPO11 (CT) may be associated with idiopathic male infertility in ethnic Han Chinese.
