Application of fluorescent in situ hybridization in primary diagnosis of aplastic anemia by morphology.
- Author:
Xiaolan LIN
1
;
Wanzi CHEN
;
Qiang FU
;
Jiadi CHEN
;
Huifang HUANG
Author Information
- Publication Type:Journal Article
- MeSH: Adolescent; Adult; Aged; Anemia, Aplastic; diagnosis; genetics; Bone Marrow Cells; cytology; Child; Chromosome Aberrations; Chromosomes, Human, Pair 7; genetics; Chromosomes, Human, Pair 8; genetics; Female; Humans; In Situ Hybridization, Fluorescence; methods; Karyotyping; Male; Middle Aged; Trisomy; genetics
- From: Chinese Journal of Medical Genetics 2016;33(1):5-8
- CountryChina
- Language:Chinese
-
Abstract:
OBJECTIVETo investigate the clinical application of fluorescent in situ hybridization (FISH) for the differential diagnosis of myelodysplastic syndromes (MDS) and aplastic anemia (AA).
METHODSA FISH kit capable of detecting the chromosomal abnormalities related to MDS was used to analyze 94 patients who were suspected to have AA by bone marrow morphology.
RESULTSCytogenetic abnormalities were detected in 11 of the 94 patients, which included trisomy 8 (5 cases), 20q- (1 case) and -Y (1 case). There were 4 cases related to MDS, which included 3 cases of 5q-, in which 1 case carry 20q- at the same time, and 7q- (1 case). No significant difference was found between the MDS and AA groups in terms of age, sex or routine blood examination including absolute neutrophil count, hemoglobin content and platelet count.
CONCLUSIONFISH can detect certain cytogenetic abnormalities related to MDS in patients morphologically diagnosed as AA.
