Application of eight-probe fluorescence in situ hybridization and R-banding karyotype analysis for the diagnosis of acute lymphoblastic leukemia.
- VernacularTitle:八探针荧光原位杂交联合R显带技术诊断儿童急性淋巴细胞白血病
- Author:
Ding ZHAO
1
;
Shuai LIU
;
Zhenxin GUO
;
Rui LI
Author Information
- Publication Type:Journal Article
- MeSH: Chromosome Aberrations; Chromosome Banding; methods; Genetic Testing; methods; Humans; In Situ Hybridization, Fluorescence; methods; Karyotyping; methods; Precursor Cell Lymphoblastic Leukemia-Lymphoma; diagnosis; genetics
- From: Chinese Journal of Medical Genetics 2016;33(1):9-12
- CountryChina
- Language:Chinese
-
Abstract:
OBJECTIVETo assess the value of eight-probe fluorescence in situ hybridization (FISH) and R-banding karyotype analysis for the diagnosis of acute lymphoblastic leukemia (ALL).
METHODSWith the eight-probe FISH (using probes for MYC, P16, E2A, CHIC2/D10Z1/D17Z1, TEL/AMLl, MLL, BCR/ABL1, and IGH) and R-banding karyotype analysis, 237 cases of ALL were analyzed.
RESULTSCytogenetic changes were detected in 135 (56.96%) of all cases, which have involved MYC, P16, E2A, CHIC2/D10Z1/D17Z1, TEL/AMLl, MLL, BCR/ABL1, and IGH polyploidies. R-banding karyotype analysis has only detected abnormalities in 48 of such cases, in addition with 14 abnormalities missed by the FISH probes, which have given a total positive rate of 26.16%. The detection rate of the two methods has differed significantly(P<0.05).
CONCLUSIONCompared with the R-banding karyotype analysis, the eight-probe FISH is more accurate and efficient. Diagnosis of cytogenetic abnormalities for children with ALL using the combined method can provide a basis for evaluation of prognosis as well as personalized therapy.
