Functional genomics studies of Salvia miltiorrhiza III. analyze of metallothionein (MT-2) genes.
- Author:
Guang-Hong CUI
1
;
Ying MAO
;
Lu-Qi HUANG
;
Yuan YUAN
;
Xue-Yong WANG
Author Information
- Publication Type:Journal Article
- MeSH: Amino Acid Sequence; Base Sequence; DNA, Complementary; genetics; Gene Expression Regulation, Plant; Genes, Plant; Genomics; Metallothionein; genetics; metabolism; Molecular Sequence Data; Oligonucleotide Array Sequence Analysis; Open Reading Frames; Plants, Medicinal; genetics; metabolism; Salvia miltiorrhiza; genetics; metabolism; Sequence Analysis, DNA; Sequence Homology, Amino Acid
- From: China Journal of Chinese Materia Medica 2007;32(14):1393-1395
- CountryChina
- Language:Chinese
-
Abstract:
OBJECTIVETo study the metallothionein genes of Salvia miltiorrhiza through bioinformatics and characterization of tissue expression in regenerated shoots.
METHODMetallothionein genes were obtained by cDNA microarray analyze. BLAST was used for align, ORF finder software was used to find open reading frame, Prosite database was used to analyze the protein. Semi-quantitative RT-PCR method was used to detect the gene expression level.
RESULTTwo metallothionein genes were obtained which were contained a deduced amino acid sequence of 80 and 79 residues, named as SmMT-2a and SmMT-2b, they had a homology of 71.25%. Semiquantitative RT-PCR indicated that metallothionein genes were expressed in all tissues such as root, stem and leaf in regenerated shoots, while the expression level was higher in leaf than in root and stem.
CONCLUSIONIt was the first time that metallothionein genes were obtained from S. miltiorrhiza. It provides a good basis for further functional study of S. miltiorrhiza.
