Simultaneous determination of geniposide, baicalin and berberine hydrochloride in Angong Niuhuang pill by RP-HPLC.
- Author:
Feng-mei HAN
1
;
Yu LI
;
Lu-jun LI
;
Yong CHEN
Author Information
- Publication Type:Journal Article
- MeSH: Berberine; analysis; Chromatography, High Pressure Liquid; methods; Drug Combinations; Drugs, Chinese Herbal; chemistry; isolation & purification; Flavonoids; analysis; Iridoids; analysis; Materia Medica; chemistry; Plants, Medicinal; chemistry; Pyrans; analysis
- From: China Journal of Chinese Materia Medica 2007;32(14):1406-1408
- CountryChina
- Language:Chinese
-
Abstract:
OBJECTIVEA new method for the simultaneous quantitative determination of geniposide, baicalin and berberine hydrochloride in Angong Niuhuang pill using reversed phase high performance liquid chromatographic method had been developed.
METHODThe optimum chromatographic conditions were as follows: Agilent Zorbax SB - C18 column (4.6 mm 250 mm, 5 m), acetonitrile-H2O (containing 6 mmol L(-1) KH2PO4, pH 4.6) gradient elution; as a detection wavelength of 343 nm.
RESULTThe calibration curves of geniposide, baicalin and berberine were linear at the ranges of 4.50-110.00, 5.00-153.00, 6.40-191.00 mg L(-1), respectively. The limits of detection of the method were 0.77 ng for geniposide, 1.53 ng for baicalin and 1.43 ng for berberine hydrochloride. The recoveries of the method were 104.44% (RSD 1.79% ) for geniposide, 96.98% (RSD 1.76%) for baicalin, 101.08% (RSD 3.1%) for berberine hydrochloride.
CONCLUSIONThis method had been successfully applied to determine the content of geniposide, baicalin and berberine hydrochloride in Angong Niuhuang pill.
