Determination of citrinin in red kojic by HPCE.
- Author:
Jun CHEN
1
;
Zhang-wan LI
;
Wei QI
;
Zhong-rong LIU
;
Bo-gang LI
Author Information
- Publication Type:Journal Article
- MeSH: Citrinin; analysis; Electrophoresis, Capillary; methods; Hydrogen-Ion Concentration; Monascus; chemistry; Reproducibility of Results
- From: China Journal of Chinese Materia Medica 2007;32(14):1412-1415
- CountryChina
- Language:Chinese
-
Abstract:
OBJECTIVETo establish an instant determination method of citrinin in red kojic by high performance capillary electrophorphores for the first time.
METHODRed kojic was extracted with the mixtrue of Toluene and ethyl acetate (70:30). Separation was carried out in an uncoated fused silica capillary (50 microm x 45.0 cm). Meanwhile, a running voltage 15 kV, 20 mmol L(-1) borax buffer with 10.0 mmol L(-1) sodium deoxycholate (pH 9.3) and a UV detector at 212 nm were adopted.
RESULTRegression equation of citrinin was Y=9434 + 16781X (r =0.990), The lower limit of quantification (S/N > or =3) was 0.5 mg mL(-1). The assay coefficients of variation ranged from 98.8% to 101.1%. The intra and inter recovery ranged from 0.83 to 1.54% and from 1.86 to 5.09%. Twenty samples were determined with the method.
CONCLUSIONThe method is proved to be simple, rapid and accurate, and it can be used to determine citrinin in red kojic.
