Study on RP-HPLC fingerprint of xinshao injection.
- Author:
Hong SU
1
;
Ai-min WANG
;
Yong-lin WANG
;
Yan-yu LAN
;
Xun HE
;
Yong-jun LI
Author Information
- Publication Type:Journal Article
- MeSH: Chromatography, High Pressure Liquid; methods; Drug Combinations; Drugs, Chinese Herbal; administration & dosage; chemistry; Erigeron; chemistry; Injections; Paeonia; chemistry; Plants, Medicinal; chemistry; Quality Control; Reproducibility of Results
- From: China Journal of Chinese Materia Medica 2007;32(15):1515-1517
- CountryChina
- Language:Chinese
-
Abstract:
OBJECTIVETo establish the fingerprint detecting standard of xinshao Injection by reversed phase high performance liquid chromatography.
METHODThe chromatographic conditions were as follows: an Diamonsil C18 column was used, the mobile phase was composed of 80% acetontrile and 0.1% phosphoric acid with gradient elution, the flow rate 1.0 mL min(-1), column temperature was 40 degrees and the UV absorbance detection was set at 230 nm.
RESULTA standard HPLC fingerprint procedure was developed for xinshao Injection, with 8 common peaks. Under the same selected chromatographic conditions, the HPLC fingerprints of raw drugs and their intermediate, finished products were obtained with good separation and correlation (no less than 0.97) according to the technical requirements of fingerprint on Injection of Chinese traditional medicine.
CONCLUSIONThis method was accurate, repeatable and can be used as the important parameters of the quality control for xinshao injection.
