AFLP analysis for genetic diversity of Ganoderma.
- Author:
Lin-yong ZHENG
1
;
Ding-hong JIA
;
Xia LUO
;
Zhi-rong YANG
Author Information
- Publication Type:Journal Article
- MeSH: Amplified Fragment Length Polymorphism Analysis; China; Cluster Analysis; DNA Primers; DNA, Fungal; analysis; genetics; Ganoderma; classification; genetics; Genetic Variation; Phylogeny; Reishi; genetics
- From: China Journal of Chinese Materia Medica 2007;32(17):1733-1736
- CountryChina
- Language:Chinese
-
Abstract:
OBJECTIVETo investigate the genetic diversity of Ganoderma cultivars provided for the genuineness study, germ-plasm resource identification, genetic relationship study, breeding, introduction and cultivante of Ganoderma strains.
METHODWith the software of NTSYSpc 2. 1, 24 materials, of G. lucidum and G. sinense, were studied using AFLP to construct the dendrogram.
RESULTThere were 177 polymorphic bands with 14 primer combinations. And all materials could be identified with AFLP.
CONCLUSIONThere actually existed much genetic diversity at the molecular level among the germplasm resources of Ganoderma strains, and all the strains were clustered into G. lucidum group and G. sinense group at the similarity coefficient 0. 676.