Determination of three alkaloids in total alkaloids exfracts from Lotus plumule by RP-HPLC.
- Author:
Li-bo ZHAO
1
;
You JIN
;
Jia-ling WANG
Author Information
- Publication Type:Journal Article
- MeSH: Alkaloids; chemistry; isolation & purification; Benzylisoquinolines; analysis; Chromatography, High Pressure Liquid; methods; Drugs, Chinese Herbal; chemistry; isolation & purification; Isoquinolines; analysis; Loteae; chemistry; Phenols; analysis; Plants, Medicinal; chemistry; Reproducibility of Results; Seeds; chemistry
- From: China Journal of Chinese Materia Medica 2007;32(17):1768-1770
- CountryChina
- Language:Chinese
-
Abstract:
OBJECTIVETo develop a RP-HPLC method for determination of three alkaloids in total alkaloids exfracted from Lotus plumule.
METHODA Hypersil C18 column was used with the mobile phase of methanol-phosphate (73:27, pH 9.0) at the detection wavelength of 230 and 286 nm. The flow rate was 1 mL min(-1).
RESULTThe linear range was 20-700 microg mL(-1), the recovery of three alkaloids were 97.9%, 98.4% and 98.1%. The RSD of intra-day and inter-day was 0. 24% -4. 83%. Different groups of samples were determined by this method. The result showed that the extraction method with methanol was prior to the extraction method with alcohol.
CONCLUSIONThe method is simple, rapid and suitable for the determination of three alkaloids in total alkaloids exfracted from L. plumule.
