Effects of resveratrol on inflammatory process induced by focal cerebral ischemia-reperfusion in rats.
- Author:
Yong-gang LIU
1
;
Xiao-dong WANG
;
Xiao-bing ZHANG
Author Information
- Publication Type:Journal Article
- MeSH: Animals; Blood-Brain Barrier; drug effects; Brain; drug effects; metabolism; pathology; Brain Ischemia; complications; Infarction, Middle Cerebral Artery; blood; etiology; prevention & control; Inflammation Mediators; blood; Interleukin-1beta; blood; Interleukin-6; blood; Male; Neuroprotective Agents; pharmacology; Peroxidase; metabolism; Random Allocation; Rats; Rats, Sprague-Dawley; Reperfusion Injury; complications; Stilbenes; pharmacology; Tumor Necrosis Factor-alpha; metabolism
- From: China Journal of Chinese Materia Medica 2007;32(17):1792-1795
- CountryChina
- Language:Chinese
-
Abstract:
OBJECTIVETo investigate the protective effects of resveratrol on inflammatory process induced by focal cerebral ischemia-reperfusion in rats.
METHODRats were pretreated with resvreratrol at the dose of 10, 20, 40 mg kg(-1) for 7 days and then subjected to cerebral ischemia/reperfusion induced by a middle cerebral artery occlusion (MCAO). The infarct volume and the neurological deficit were determined by the method of TTC (2, 3, 5-triphenylterazolium chloride) staining and Longa's score. The permeability of blood-brain barrier (BBB) was evaluated by measurement of the evans blue (EB) content in the brain with spectrophotometer. The content of interleukin-lbeta, interleukin-6 (IL-6, IL-1beta) in serum and tumor necrosis factor-alpha (TNF-alpha), myeloperoxidase (MPO) in brain were determined by radio-immunoassay and ELISA assay.
RESULTResveratrol reduced infarct volume, ameliorated the neurological deficit and the permeability of BBB, the content of IL-6, IL-1beta in serum and TNF-alpha, MPO activity in brain tissue also were significantly decreased.
CONCLUSIONThese results showed that resveratrol had protective effects on cerebral injury by inhibiting the releasing of the inflammatory mediators after ischemia/reperfusion injury.
