Observation of pollen tube germination of Platycodon grandiflorum by fluorescence microscopy.
- Author:
Jian-he WEI
1
;
Feng-jie MA
;
Lu-qi HUANG
;
Cheng-min YANG
;
Zhi-tu SUN
;
Qi-ting DONG
;
Ai-min CAI
Author Information
- Publication Type:Journal Article
- MeSH: Flowers; growth & development; Microscopy, Fluorescence; methods; Plants, Medicinal; growth & development; Platycodon; growth & development; Pollen Tube; growth & development; Pollination; physiology
- From: China Journal of Chinese Materia Medica 2007;32(16):1625-1627
- CountryChina
- Language:Chinese
-
Abstract:
OBJECTIVETo determine the self-compatibility of Platycodon grandiflorum and the location of microspore germination on stigma.
METHODThe microspore germination of self-pollination, self-plant-pollination and cross-pollination and the pollination microspore germination in and outside of stigma were observed with fluorescence microscopy.
RESULTMost pollens of self-pollination, self-plant-pollination and cross-pollination can germinate on the stigma, and after 24 hours, pollen tube entered into the ovary successfully. Pollinated on the outer-surface of stigma, microspores could not germinate, but on the inner-surface of the stigma when it dehisced most microspores can germinate.
CONCLUSIONThe compatibility of self-plant-pollination of Platycodon grandiflorum is high. The microspore germination loci is on the inner-surface of the dehisced stigma.
