Simultaneous determination of peimine, peiminine and zhebeinine in Fritillaria thunbergii from different habitat by HPLC-ELSD.
- Author:
Yan XUE
1
;
Feng WANG
Author Information
- Publication Type:Journal Article
- MeSH: Cevanes; analysis; China; Chromatography, High Pressure Liquid; instrumentation; methods; Ecosystem; Fritillaria; chemistry; growth & development; Light; Plants, Medicinal; chemistry; growth & development; Reproducibility of Results; Scattering, Radiation
- From: China Journal of Chinese Materia Medica 2007;32(16):1628-1630
- CountryChina
- Language:Chinese
-
Abstract:
OBJECTIVETo determine peimine, peiminine and zhebeinine in Fritillaria thunbergii simultaneously by RP-HPLC-ELSD.
METHODThe HPLC was carried out with a Waters Alliance, Model 2690, equipped with an Xterra RP18 column (3.9 mm x 150 mm, .5 microm), and evaporated light scattering detector. It was eluted gradiently with acetonitrile and 10 mmol x L(-1) NH4HCO3 (adjusted to pH 10. 10 by ammonia solution).
RESULTThe average recovery rates (n=5) of peimine, peiminine and zhebeinine were 98.2% (RSD 1.7%), 98.2% (RSD 2.2%), 96.5% (RSD 1.3%) respectively.
CONCLUSIONThe method is simple, sensitive and reliable. It can be used for quantitative determination of F. thunbergii.
