Determination of chyodeoxycholic acid in Bile Arisaema by HPLC-ELSD.
- Author:
Yan HE
1
;
Yong-xin ZHANG
;
Hui-dong ZHAO
;
Qi-wei ZHANG
Author Information
- Publication Type:Journal Article
- MeSH: Animals; Arisaema; chemistry; Bile; chemistry; Chromatography, High Pressure Liquid; instrumentation; methods; Deoxycholic Acid; analysis; Drug Combinations; Hot Temperature; Light; Medicine, Chinese Traditional; standards; Plant Tubers; chemistry; Plants, Medicinal; chemistry; Quality Control; Reproducibility of Results; Scattering, Radiation
- From: China Journal of Chinese Materia Medica 2007;32(16):1634-1636
- CountryChina
- Language:Chinese
-
Abstract:
OBJECTIVETo develop an HPLC method for the determination of hyodeoxycholic acid in Bile Arisaema.
METHODThe methanol extracts were separated on a Diamonsil ODS column eluted at 35 degrees C with a mixture of methanol-water-glacial acetic acid (75:25:0.01) at flow-rate 1.0 mL x min(-1). The drift-tube temperature of ELSD was 85 degrees C, and the flow rate of air was 3.0 L x min(-1).
RESULTHyodeoxycholic acid in methanol extract was well separated, the relationship of logarithms of injection amount and peak area was linear (r = 0.9998) over the range of 2.4-24 microg. The average recovery was 97.1% (RSD 2.9%, n=5).
CONCLUSIONThe method could be used for quality control of Bile Arisaema.
