Determination of tenuifolin in Tianwang Buxinwan and Guipiwan by HPLC.
- Author:
Xiao-bing DONG
1
;
Jun LI
;
Yong JIANG
;
Ying ZHENG
;
T X Tina DONG
;
Peng-fei TU
;
W K Karl TSIM
Author Information
- Publication Type:Journal Article
- MeSH: Chromatography, High Pressure Liquid; methods; Codonopsis; chemistry; Diterpenes, Kaurane; analysis; Drug Combinations; Drugs, Chinese Herbal; chemistry; Plants, Medicinal; chemistry; Polygala; chemistry; Reproducibility of Results; Salvia miltiorrhiza; chemistry; Saponins; analysis; Triterpenes; analysis
- From: China Journal of Chinese Materia Medica 2007;32(16):1647-1649
- CountryChina
- Language:Chinese
-
Abstract:
OBJECTIVEA quantitative method was developed for the determination of tenuifolin in Tianwang Buxinwan and Guipiwan by HPLC.
METHODThe samples were separated by Alltima C18 column (4.6 mm x 250 mm, 5 microm) using methanol--0.05% phosphoric acid (65:35) as a mobile phase, flow rate was 1.0 mL x min(-1) and wavelength was set at 202 nm.
RESULTTenuifolin was detected in both Chinese preparations. The number of theoretical plates calculated by tenuifolin peak was 2 500. The regression equation of tenuifolin was Y = 5.239 x 10(6) X-6.247 x 10(5) (r = 0.9994) and the liner range was 10-500 g x mL(-1). The average recovery of tenuifolin was 97.5% (RSD less than 3.0%). The LOD of tenuifolin was 5.50 g x mL(-1).
CONCLUSIONThe method is sensitive, rapid and accurate.
