Study on the distribution of sub-genotype B on hepatitis B virus in patients with chronic HBV infection from 4 cities of China.
- Author:
Jie WANG
1
;
Jun-wei GAO
;
Hui ZHUANG
;
Jie LI
;
Jia WANG
;
Ya-juan LI
;
Hui JIN
Author Information
- Publication Type:Journal Article
- MeSH: China; Genotype; Hepatitis B; virology; Hepatitis B virus; classification; genetics; Humans; Polymerase Chain Reaction; Polymorphism, Restriction Fragment Length; genetics
- From: Chinese Journal of Epidemiology 2008;29(1):52-54
- CountryChina
- Language:Chinese
-
Abstract:
OBJECTIVETo investigate the distribution of sub-genotype B on hepatitis B virus (HBV) in patients with HBV chronic infection from 4 cities (Beijing, Shijiazhuang, Wenzhou and Shenzhen) of China.
METHODSA type-specific nested polymerase chain reaction(PCR) with multiplex pairs of primers was used for HBV genotyping,and Ba and Bj sub-genotypes were identified by a PCR-restriction fragment length polymorphism (PCR-RFLP) method. A total of 101 serum samples collected from patients with chronic HBV/B infection were detected. Among them, 18 were collected from Beijing, 22 from Shijiazhuang, 34 from Wenzhou and 27 from Shenzhen. Thirty from the 101 serum samples were randomly selected and analyzed by PCR product sequencing.
RESULTSAll of the 101 serum samples were identified as sub-genotype Ba,and none of them was sub-genotype Bj.
CONCLUSIONSub-genotype Ba was a predominant strain of HBV/B in patients with chronic HBV infection from these 4 cities.
