Study on the molecular epidemiology of Hantavirus carried by rodent hosts in Cixi, Zhejiang province
10.3321/j.issn:0254-6450.2008.04.013
- VernacularTitle:浙江省慈溪市啮齿动物中汉坦病毒分子流行病学研究
- Author:
Fei-Neng FAN
1
;
Peng-Yei YANG
;
Nan-Feng SHI
;
Na GAO
;
Guo-Hua CHEN
;
Ming-Hui LI
;
Yang ZOU
;
Xiao-Zhao DENG
;
Yong-Zhen ZHANG
Author Information
1. 浙江省慈溪市疾病预防控制中心
- Keywords:
Hantavirus;
Seonl virus;
Phylogenetic analysis;
Genotype
- From:
Chinese Journal of Epidemiology
2008;29(4):365-368
- CountryChina
- Language:Chinese
-
Abstract:
Objective To collect more data on the epidemiology of hantavirus in rodents in Cixi,Zhejiang province.Methods Rodents were captured in Cixi,where hemorrhagic fever with renal syndrome (HFRS)appeared endemic.Hantavirus antigens in the rat lungs were detected by immunofluorescence assay.Partial S segment sequences were amplified by reverse transciption-polymerase chain reaction(RTPCR)and then sequenced.The phyologenetic trees were constructed by maximum likelihood method to detect the genetic characteristics of hantavirus.Results A total of 243 rodents were trapped in the epidemic areas,and hantavirus antigens were identified in 7 out of these lung samples(2.88%).Partial S segment sequences(620-999 nt)were recovered from 6 samples and sequenced.Data from phylogenetic analysis of these S segment sequences indicated that all viruses belonged to Seoul virus(SEOV),despite the origins of sources were either from Rattus norvegicus or from R.fzabipectus.These viruses could further be divided into two distinct lineages but the viruses carried by R.norvegicus were different from those carried by R.flabipectus.Conclusion Two distinct lineages of SEOV had been cocirculating in rodents in Cixi.
