Human LINE1 endonuclease domain as a putative target of SARS-associated autoantibodies involved in the pathogenesis of severe acute respiratory syndrome.
- Author:
Wei-ping HE
1
;
Cui-li SHU
;
Bo-an LI
;
Jun ZHAO
;
Yun CHENG
Author Information
- Publication Type:Journal Article
- MeSH: Antibodies, Viral; immunology; Autoantibodies; immunology; Endonucleases; immunology; Gene Library; Humans; Long Interspersed Nucleotide Elements; immunology; Polymerase Chain Reaction; SARS Virus; immunology; Severe Acute Respiratory Syndrome; etiology
- From: Chinese Medical Journal 2008;121(7):608-614
- CountryChina
- Language:English
-
Abstract:
BACKGROUNDSevere acute respiratory syndrome (SARS) is a disease with a mortality of 9.56%. Although SARS is etiologically linked to a new coronavirus (SARS-CoV) and functional cell receptor has been identified, the pathogenesis of the virus infection is largely unclear.
METHODSThe clinical specimens were processed and analyzed using an indirect enzyme-linked immunosorbent assay (ELISA) in-house. Further investigations of target antigen included reviews of phage display technique, rapid amplification of cDNA ends (RACE) technique, protein expression and purification, Western blotting validation, serological and immunohistochemical staining in postmortem tissue.
RESULTSA type of medium or low titer anti-lung tissue antibodies were found in the sera of SARS patients at the early stage of the disease. Human long interspersed nuclear element 1 (LINE1) gene endonuclease (EN) domain protein was one of the target autoantigens and it was aberrantly expressed in the lung tissue of SARS patients. Anti-EN antibody was positive in the sera of 40.9% of SARS patients.
CONCLUSIONSHuman LINE1 endonuclease domain was identified as a putative target of SARS-associated autoantibodies, which were presented in the serum of SARS patients and may be involved in the pathogenesis of SARS.
