Research wilt disease of Salvia miltiorrhiza and its pathogen.
- Author:
Li YANG
;
Zuo-Qing MIAO
;
Guang YANG
;
Ai-Juan SHAO
;
Lu-Qi HUANG
;
Ye SHEN
;
Xue WANG
;
Mei-Lan CHEN
- Publication Type:Journal Article
- MeSH:
DNA, Intergenic;
genetics;
Fusarium;
genetics;
isolation & purification;
physiology;
Plant Diseases;
microbiology;
Polymerase Chain Reaction;
Salvia miltiorrhiza;
microbiology;
Seasons
- From:
China Journal of Chinese Materia Medica
2013;38(23):4040-4043
- CountryChina
- Language:Chinese
-
Abstract:
Salvia miltiorrhiza is a highly valued traditional chinese medicine for the treatment of atherosclerosis-related disorders in china, such as cardiovascular and cerebrovascular diseases in China. The wilt disease is serious in the culture of S. miltiorrhiza. Wilt disease cause biomass of plant shoots and roots is lessened, active components are decreased. To solve these problems, we research the pathogen causing wilt disease of S. miltiorrhiza. The suspected pathogen is identified by morphology and etiological test. The identification was further confirmed by alignment the sequences of internal transcribed spacer (ITS) amplified by PCR. Our result show the wilt disease of S. miltiorrhiza mostly occurred in July and August, which is hot and wetter. The wilt disease rate of S. miltiorrhiza continuous cropping for one year in S. miltiorrhiz stubble is 10%, but the wilt disease rate of S. miltiorrhiza continuous cropping for three years in S. miltiorrhiz stubble is 60%-70%. The root rot of S. miltiorrhiz caused by the wilt disease, so the wilt disease was mistaken for the rot root in production. Morphological characteristics show the pathogen is Fusarium oxysporum. The sequence of ITS wes determined and found by BLAST shared 99% identity to that of F. oxysporum f. sp. cucumerinum. So it comes to the conclusion that the causing agent of wilt disease on S. miltiorrhiza belongs to F. oxysporum.
