Analysis on biotransformation of Epimedium brevicornu flavonoids.
- Author:
Xia GAO
;
Xuan LIU
;
Yan CHEN
;
Ying WANG
;
Xiao-Bin JIA
- Publication Type:Journal Article
- MeSH:
Biotransformation;
Epimedium;
chemistry;
Flavonoids;
metabolism;
Hydrogen-Ion Concentration;
Hydrolases;
metabolism;
Hydrolysis;
Temperature
- From:
China Journal of Chinese Materia Medica
2013;38(23):4079-4083
- CountryChina
- Language:Chinese
-
Abstract:
This study aims to investigate the biotransformation of Epimedium brevicornu flavonoids under the effect of hydrolytic enzymes in vitro. Snailase was mainly used to hydrolyze E. brevicornu flavonoids, and HPLC was used to determine the content of the main flavonoids in E. brevicornu flavonoids. The data results showed that the main known flavonoids included icariin, epimedin A, epi-mendin B and epimendin C, which were completely transformed into baohuoside I, sagittatoside A, sagittatoside B and 2"-O-rhamnosyl-icariside II in 1-2 h, respectively. Their transformed products were continuously hydrolyzed over time. In conclusion, snailase could transform E. brevicornu flavonoids into secondary glycoside or aglycone under 37 degrees C in pH 6.0 HBSS balanced salt solution in 2 h. Moreover, its enzymatic hydrolysates were consistent with intestinal metabolites.
