Difference in effect between asthma-based mouse model and Stemona tuberosa extracts.
- Author:
Xiao-Xi CHEN
;
Xiao-Dan ZHANG
;
Hong-Yan LI
;
Tian-Zhu JIA
;
Jing-Xian YANG
- Publication Type:Journal Article
- MeSH:
Administration, Oral;
Animals;
Asthma;
drug therapy;
immunology;
metabolism;
Disease Models, Animal;
Drugs, Chinese Herbal;
administration & dosage;
pharmacology;
therapeutic use;
GATA3 Transcription Factor;
metabolism;
Gene Expression Regulation;
drug effects;
Mice;
Mice, Inbred BALB C;
Stemonaceae;
chemistry;
T-Box Domain Proteins;
metabolism;
Th1 Cells;
drug effects;
secretion;
Th2 Cells;
drug effects;
secretion
- From:
China Journal of Chinese Materia Medica
2013;38(23):4084-4087
- CountryChina
- Language:Chinese
-
Abstract:
In this study, OVA-induced asthma mice was taken as the model, and orally administered with different concentration of ethanol extracts of crude and processed Stemona tuberosa, in order to determine the cytokine level released from Th1 and Th2 in splenocytes. RT-PCR was carried out to determine the genetic expression of T-bet/GATA-3 in lung, and compare the differentiation between ethanol extracts of crude and processed S. tuberosa in therapeutic effect on asthma in mice. According to the results, compared with the crude samples, processed samples significantly increased the levels of inflammatory factor INF-gamma (P < 0.05) and decreased IL-5 (P < 0.05) in splenocytes. According to the RT-PCR results, the administration of processed samples could increase the ratio of T-bet/GATA-3 (P < 0.05). The experiment showed that ethanol extracts of both crude and processed S. tuberosa could treat asthma by regulating Th1/Th2 ratio, but processed samples showed more notable effect. This indicated that crude and processed S. tuberosa had significant pharmacological difference. Therefore, it was more rational to apply processed S. tuberosa in clinical treatment of asthma and chronic cough, which layed a foundation for further revealing the processing mechanism of S. tuberosa.
