Antitussive constituents of Disporum cantoniense.
- Author:
Xiu-Hai GAN
;
Chao ZHAO
;
Zhi-Yuan LIANG
;
Xiao-Jian GONG
;
Hua-Guo CHEN
;
Xin ZHOU
- Publication Type:Journal Article
- MeSH:
Animals;
Antitussive Agents;
chemistry;
isolation & purification;
pharmacology;
Drugs, Chinese Herbal;
chemistry;
isolation & purification;
pharmacology;
Ethanol;
chemistry;
Female;
Liliaceae;
chemistry;
Male;
Mice
- From:
China Journal of Chinese Materia Medica
2013;38(23):4099-4103
- CountryChina
- Language:Chinese
-
Abstract:
The antitussive activity assay for the root extraction of Disporum cantoniense was carried out with coughing mice induced by ammonia liquor. The results showed that the ethanol and water extractions of D. cantoniense possess strong antitussive activity, and the high dose of the former was better than positive control, and then the constituents of the ethanol extraction were separated and purified by various modern chromatographic techniques. Their structures were identified by physico-chemical properties and spectroscopic data. As a result, eight compounds were isolated and identified as stigmast-4-en-3-one(1), (22E, 24R)-ergosta-5, 7, 22-trien-3beta-ol(2), obtucarbamate A(3), obtucarbamate B(4), neotigogenin(5), azo-2, 2'-bis[Z-(2,3-dihydroxy-4-methyl-5-methoxy) phenyl ethylene] (6),dimethyl {[carbonylbis (azanediyl)] bis( 2-methyl-5, 1-phenylene) j dicarbamate (7) , and quercetin-3-O-pB-D-glucopyranoside(8). All compounds were isolated from this plant for the first time, and the result of bioactivity-directed isolation showed that compounds 3, 4, and 6 had obvious effect on antitussive activity, and compound 6 had the same level as positive control.
