Studies on quality evaluation of Dendrobii devoniani.
- Author:
Xiao-Na GAN
;
Ying XU
;
Hong XU
;
Jia-Bao LIU
;
Li YANG
;
Zheng-Tao WANG
- Publication Type:Journal Article
- MeSH:
Chromatography, High Pressure Liquid;
Dendrobium;
chemistry;
Drugs, Chinese Herbal;
chemistry;
Polysaccharides;
analysis;
Quality Control
- From:
China Journal of Chinese Materia Medica
2013;38(23):4113-4118
- CountryChina
- Language:Chinese
-
Abstract:
To establish the local quality standard for Dendrobii devoniani caulis from Longling, Yunnan, the pharmacognostic characteristics microscopic characteristics and TLC identification were developed. Sulfuric acid-phenol method was used to determine the content of polysaccharide. An HPLC method was adopted to determine the content of mannose, and extractives were determined according to the procedures recorded in the Appendix of Chinese Pharmacopoeia(2010). The results showed a strong characteristics microscopic of Dendrobii devoniani caulis, and its TLC identification had a good resolution with clear spots; the content of polysaccharide is 35.7% -52.1% (average 42.7%), mannose 27.8%-46.1% (average 35.8%), and extract 4.5%-10.6% (average 7.38%). The method is simple, accurate and reliable, with good reproducibility. The established standard is acceptable for quality evaluation of Dendrobii devoniani caulis from Longling, Yunnan.
