Determination of dactylorhin A and militarine in three varieties of Cremastrae Pseudobulbus/Pleiones Pseudobulbus by HPLC.
- Author:
Bao-Song CUI
;
Jie SONG
;
Shuai LI
;
Lin MA
;
Jian-Gong SHI
- Publication Type:Journal Article
- MeSH:
Chromatography, High Pressure Liquid;
Glucosides;
analysis;
Orchidaceae;
chemistry;
Succinates;
analysis
- From:
China Journal of Chinese Materia Medica
2013;38(24):4347-4350
- CountryChina
- Language:Chinese
-
Abstract:
To establish an HPLC method for determination of dactylorhin A and militarine in Cremastrae Pseudobulbus/Pleiones Pseudobulbus. The analysis was achieved on an Alltech Prevail C18 column (4. 6 mm x 250 mm, 5 microm) using a mobile phase of acetonitrile (A), water (B) gradient elution in a total run time of 35 min (0 min, 20:80; 30 min, 55:45; 35 min, 55:45) and a diode array detector was set at 224 nm. The flow rate was 0.8 mL x min(-1). The assay displayed good linearity over the concentration range of 0.257-9.95 microg (r = 0.999 8), and 0.128-10.27 microg (r = 0.999 9), respectively. The average recoveries (n = 9) were 94.70% and 102.8% for dactylorhin A and militarine, respectively. The method is accurate, quick, simple and reproducibility. It can be used for the quality control of Pleione bulbocodioides and Pleione yunnanensis.