Identification of constituents in Suanzaoren tang by LC-Q-TOF-MS and LC-IT-MS.
- Author:
Yun-Xiang ZHU
;
Lu-Lin CHEN
;
Jing-Ru GONG
;
Shu-Fang WANG
- Publication Type:Journal Article
- MeSH:
Chromatography, Liquid;
Drugs, Chinese Herbal;
chemistry;
Mass Spectrometry;
Organic Chemicals;
analysis
- From:
China Journal of Chinese Materia Medica
2014;39(3):457-465
- CountryChina
- Language:Chinese
-
Abstract:
LC-Q-TOF-MS and LC-IT-MS in positive and negative ion mode were applied to simultaneously characterize the constituents in Suanzaoren tang. Analysis was performed on an Agilent Zorbax SB-C18, Rapid Resolution HT column(4.6 mmx 50 mm, 1. 8 micro m) with gradient elution of acetonitrile(A) -aqueous solution containing 0. 05% formic acid(B) at a flow rate of 0. 6 mL min(-1) and the column temperature was 30 degreesC. By comparing MS fragmentation, accurate molecular weight, literature date and standard compounds information, a total of48 compounds were successfully identified or speculated. The origins of these compounds were assigned to the corresponding Chinese medicine. Thirty-one compounds were reported in Suanzaoren tang for the first time. LC-Q-TOF-MS combined with LC-IT-MS is a simple and rapid tool for the identification of constituents of Suanzaoren tang, and the results could provide evidence for the research on quality combined and effective constituents of Suanzaoren tang.
