Tetrandrine reversed the resistance of tamoxifen in human breast cancer MCF-7/TAM cells: an experimental research.
- Author:
Hai-yan CHEN
1
;
Xin-yi CHEN
Author Information
- Publication Type:Journal Article
- MeSH: Benzylisoquinolines; pharmacology; Drug Resistance, Neoplasm; drug effects; genetics; Female; Humans; MCF-7 Cells; drug effects; Multidrug Resistance-Associated Proteins; metabolism; Tamoxifen; pharmacology
- From: Chinese Journal of Integrated Traditional and Western Medicine 2013;33(4):488-491
- CountryChina
- Language:Chinese
-
Abstract:
OBJECTIVETo study the reverse effect and its mechanism of tetrandrine (Tet) on human breast cancer cells resistant to tamoxifen (MCF-7/TAM).
METHODSThe drug toxicity and the reverse effect of Tet on MCF-7/TAM cells were detected by MTT assay. The effects of multidrug resistance protein 1 (MRP1) gene of Tet on MCF-7iTAM cells were detected by real-time fluorescence quantitative PCR. The changes of MRP1 protein on MCF-7JTAM cells were detected by Western blot.
RESULTSTet had a significant reversal of drug resistance on MCF-7/TAM cells. The non-cytotoxic dose (0. 625 microg/mL) reversed the resistance by 2.0 folds. MRP1 was reduced at gene (P <0.05) and protein levels when Tet effected on MCF-7ITAM cells.
CONCLUSIONTet could reverse the drug resistance of MCF-7/TAM cells, and the reverse mechanism may be related to down-regulating MRP1 expression.
