Roles of bFGF in endothelial cell tube formation induced by xuefu zhuyu decoction under non-anoxia condition.
- Author:
Dong GAO
1
;
Jing-Si ZHANG
;
Ya-Qiong HU
;
Fan LIN
;
Yi-Zheng WANG
;
Jun SONG
;
Ke-ji CHEN
Author Information
- Publication Type:Journal Article
- MeSH: Animals; Cell Line; Drugs, Chinese Herbal; pharmacology; Endothelial Cells; drug effects; metabolism; Female; Fibroblast Growth Factor 2; metabolism; Humans; Male; Neovascularization, Physiologic; drug effects; Rats; Rats, Sprague-Dawley
- From: Chinese Journal of Integrated Traditional and Western Medicine 2013;33(5):623-626
- CountryChina
- Language:Chinese
-
Abstract:
OBJECTIVETo explore the roles of basic fibroblast growth factor (bFGF) on tube formation induced by xuefu zhuyu decoction (XZD) under non-anoxia condition.
METHODSUsing serum pharmacology technique, endothelial cell line ECV304 cells were incubated in routine 95% O2. ECV304 cells were intervened by 1.25%, 2.50%, and 5.00% XZD containing serums and the vehicle serum for 48 h. The effects of XZD on tube formation, bFGF contents and its transcription levels were assessed by in vitro tube formation assay, enzyme-linked immunosorbent assay (ELISA), and reverse transcriptase polymerase chain reaction (RT-PCR), respectively.
RESULTSThree concentrations of XZD containing serums could not only obviously promote the tube formation bFGF level, but also up-regulate bFGF contents in the supernate and its transcription levels. The shapes of lumens were more regular in those induced by 1.25% and 2.50% XZD containing serums.
CONCLUSIONXZD induced angiogenesis via up-regulating the bFGF expression under non-anoxia condition.
