Multiplex ligation-dependent probe amplification and its application.
10.3760/cma.j.issn.1003-9406.2009.01.010
- VernacularTitle:多重连接探针扩增技术及其应用
- Author:
Da-wen ZHOU
1
;
Zhao-rui REN
Author Information
1. Shanghai Institute of Medical Genetics, Shanghai Jiaotong University, Shanghai, 200040 PR China. zdwmail2006@yahoo.com.cn
- Publication Type:Journal Article
- MeSH:
DNA Methylation;
DNA Probes;
analysis;
genetics;
Humans;
Nucleic Acid Amplification Techniques;
instrumentation;
methods;
Oligonucleotide Array Sequence Analysis;
Polymorphism, Single Nucleotide
- From:
Chinese Journal of Medical Genetics
2009;26(1):45-49
- CountryChina
- Language:Chinese
-
Abstract:
Multiplex ligation-dependent probe amplification (MLPA) is a semiquantitative analysis based on polymerase chain reaction (PCR). It possesses many advantages such as high efficiency, simple operation, low cost and has been wildly applied in researches of diseases associated with copy number variation, point mutation and methylation. Recently, MLPA is combined with DNA chip to become a real high-throughput method and get great improvement in reliability. Here, the progresses of methods and application of MLPA, as well as its limitations are reviewed.
