Detection of the Philadelphia chromosome in adult B-lineage acute lymphoblastic leukemia using dual-color dual-fusion interphase fluorescence in situ hybridization.
- Author:
Tian TIAN
1
;
Si-xuan QIAN
;
Wei XU
;
Hai-rong QIU
;
Rong WANG
;
Chun QIAO
;
Ming HONG
;
Jian-fu ZHANG
;
Kou-rong MIAO
;
Jian-yong LI
Author Information
- Publication Type:Journal Article
- MeSH: Adolescent; Adult; Aged; B-Lymphocytes; metabolism; pathology; Chromosome Aberrations; Color; Female; Humans; In Situ Hybridization, Fluorescence; methods; Interphase; Karyotyping; Male; Middle Aged; Philadelphia Chromosome; Precursor Cell Lymphoblastic Leukemia-Lymphoma; genetics; pathology
- From: Chinese Journal of Medical Genetics 2009;26(1):78-81
- CountryChina
- Language:Chinese
-
Abstract:
OBJECTIVETo investigate the incidence of Philadelphia chromosome (Ph) in adult B-lineage acute lymphoblastic leukemia (B-ALL).
METHODSOne hundred and twelve adult patients with previously untreated B-ALL were prospectively investigated by interphase dual-color dual-fusion fluorescence in situ hybridization (DD-FISH) with two-color break apart probe BCR-ABL and the results were compared with that of conventional cytogenetics (CC).
RESULTSThe incidence of Ph chromosome was 17.98% (16/89) and 31.25% (35/112) by CC and DD-FISH, respectively. The mean positive rate of Ph+cells by FISH was 66.23% (ranging 18.5%-99%). Of the 35 Ph+ALL patients by FISH, 25 were successfully karyotyped by CC which included 5 normal karyotypes, 20 abnormal karyotypes including 16 Ph chromosome and 13 complex abnormalities.
CONCLUSIONThe incidence of Ph chromosome was 31.25% in adult with B-ALL. DD-FISH with BCR-ABL probe provides a powerful technique for the diagnosis of Ph+B-ALL. It is an important supplement to the CC analysis. DD-FISH technique should be used as a routine method for the diagnosis for adult acute B-ALL.
