A recombination event occurring between HLA-B and DRB1 loci within a Chinese Han family.
- Author:
Na LIU
1
;
Xiao-yan SHAN
;
Wei LI
;
Lijun WANG
;
Xiao-mei HE
;
Dong-mei WANG
;
Lei NI
;
Shuang CUI
;
Lin WANG
;
Zhi-yin GONG
;
Bo-tao ZHAO
;
Zhi-xin ZHANG
Author Information
- Publication Type:Journal Article
- MeSH: Adolescent; Asian Continental Ancestry Group; genetics; Crossing Over, Genetic; genetics; Family; Female; HLA-A Antigens; genetics; HLA-B Antigens; genetics; HLA-DQ Antigens; genetics; HLA-DQ beta-Chains; HLA-DR Antigens; genetics; HLA-DRB1 Chains; Humans; Male; Pedigree; Polymerase Chain Reaction; Recombination, Genetic
- From: Chinese Journal of Medical Genetics 2009;26(2):216-218
- CountryChina
- Language:Chinese
-
Abstract:
OBJECTIVETo investigate a recombination event occurring between the HLA-B and DRB1 loci in a Chinese family with a leukemia patient.
METHODSHLA class I (-A and -B) low resolution typing was carried out by polymerase chain reaction-sequence specific oligonucleotide, PCR-SSO). HLA class II low resolution typing was performed by PCR-sequence specific primer (PCR-SSP). And HLA class I and II high resolution typing was done by sequencing-based typing (SBT). Then the recombination event was analyzed by family study.
RESULTSThe 2 haplotypes of the patient were A*3101-B*1301-DRB1*0701 and A*3303-B*4403-DRB1*1302. His father's 2 haplotypes were A*3001-B*1302-DRB1*0701 and A*3101-B*1301-DRB1*1501. Family study demonstrated that the HLA-A*3101-B*1301 was from one of his father's chromosome and the DRB1*0701 was from the other chromosome of his father. So the result indicated that the recombination event occurred between the HLA-B and -DRB1 loci during meiosis of his father and resulted in a new HLA haplotype that was transferred to the son.
CONCLUSIONA HLA-B/DR recombination event occurring between the HLA-B and -DRB1 loci has been found in a Chinese family, which may help further study of the mechanism of HLA recombination.
