Effects of inhibition of ANG-2 expression in Ishikawa cell line.
- VernacularTitle:靶向ANG-2的shRNA重组载体抑制人子宫内膜癌Ishikawa生长和侵袭的研究
- Author:
Huai-jun ZHOU
1
;
Rong-rong HE
;
Bao-mei XIA
;
Qing-ying XUN
;
Jin-shun PAN
;
Ya-li HU
Author Information
- Publication Type:Journal Article
- MeSH: Angiopoietin-2; antagonists & inhibitors; genetics; Apoptosis; drug effects; genetics; Cell Cycle; drug effects; genetics; Cell Line, Tumor; Cell Proliferation; drug effects; Endometrial Neoplasms; genetics; pathology; Female; Gene Expression; drug effects; Gene Expression Regulation, Neoplastic; Gene Silencing; drug effects; physiology; Humans; Indicators and Reagents; Lipids; pharmacology; RNA Interference; RNA, Small Interfering; genetics; pharmacology; Transfection; Tumor Cells, Cultured
- From: Chinese Journal of Medical Genetics 2009;26(3):249-253
- CountryChina
- Language:Chinese
-
Abstract:
OBJECTIVETo investigate the effect of RNA interference mediated angiopoietin-2 (ANG-2) gene silencing on human endometrial carcinoma cell line Ishikawa.
METHODSShort hairpin RNA (shRNA) targeting ANG-2 gene was designed and transfected into Ishikawa cells with Lipofectamine 2000. The mRNA and protein expression level of ANG-2, proliferation, morphological changes, apoptosis, cell cycle and invasive ability of the cells after transfection were analyzed.
RESULTSThe shRNA targeting the human ANG-2 gene effectively decreased the expression of ANG-2 on both mRNA and protein level, the proliferation inhibition rate of the Ishikawa cells was 63.11%, cell apoptosis was induced, and the cell cycle was arrested in G1 phase. The apoptotic rate of the Ishikawa cells in the transfected group was significantly higher, and the invasive ability was decreased markedly, than that of control groups.
CONCLUSIONThe shRNA targeting human ANG-2 gene could reduce ANG-2 expression, inhibit cellular growth and invasion in Ishikawa cells in vitro.
